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Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5

Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5

Hajra Basit ,  K. Shivaji Sharma ,  Angéline Van der Heyden ,  Chantal Gondran ,  Cécile Breyton ,  Pascal Dumy ,  Françoise M. Winnik and Pierre Labbé

Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA–amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.

 

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