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Phosphorylcholine-Modified Chitosan Films as Effective Promoters of Cell Aggregation: Correlation Between the Films Properties and Cellular Response

Phosphorylcholine-Modified Chitosan Films as Effective Promoters of Cell Aggregation: Correlation Between the Films Properties and Cellular Response

B. Qi, P. Kujawa, S. Toita, G. Beaume and F. M. Winnik.

This study describes chitosan-phosphorylcholine (CH-PC) films able to support the formation of cell aggregates (spheroids), which are important for tissue engineering and pharmacological studies. The surface topography, charge, thickness, and rheology of CH-PC thin films were characterized by AFM, zeta-potential measurements, SPR spectroscopy, and QCM-D measurements. The CH-PC films are highly hydrated gels, independently of the level of PC incorporation (15–40 mol-% PC/glucosamine units). QCM-D studies established that the amount of fibrinogen adsorbed on CH-PC films decreased with increasing PC content. CH-PC surfaces underwent a transition from moderately cell-adhesive (CH-PC15) to non-adhesive (CH-PC40). Optical micrographs of HUVEC and MCF-7 cell lines cultured on CH-PC surfaces showed that they form spheroids on CH-PC25 and CH-PC40 films.

 

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